Abstract. A study was conducted to determine the variation and relationships among  papaya genotypes based on morphological  and fruit characteristics  in order to produce  high quality papaya fruits. Fruit characterization study is very useful for  genotype improvement and genotype classifi cation of papaya. In this study the morphological characters of 36 genotypes were  analyzed to determine their phenotypic variabilities.  The relationships between genotypes based on all of the morphological  and fruit characteristics were tested by subjecting the data to multivariate principal component analysis and to cluster analysis. Based on the dendrogram generated from vegetative and generative characters, the 36 genotypes could be grouped into 11 clusters on a threshold of 1.6 and formed 6 clusters on a threshold of 1.8.  The dendrogram was able to explain the  close relationship between IPB 5 x IPB 1 and IPB 5 x IPB 4, IPB 2 and IPB 7, IPB 1 and IPB 3 genotype. The scattered  diagram of generative variable divided the papaya genotypes into three groups based on fruit sizes i.e small group (IPB 1, IPB 3, IPB 4, IPB 3 x IPB 4, IPB 1 x IPB 9), medium group (IPB 5, IPB 7, IPB 8, IPB 9) and big group (IPB 2, IPB 10). The IPB 1, IPB 3 and IPB 4 were different from IPB 2 in fruit shapes, petal length of male fl owers, infl orescence size and  fruit length. The hybrid plants obtained from crossings with IPB 10 were different from the other genotypes in the colours of female-, hermaphrodite-, and male fl ower-lobes. Subsequently the scatter diagrams also revealed that several genotypes i.e. IPB 2 x IPB 6, IPB 1 x IPB 5,  IPB 1 x IPB 9, IPB 5 x IPB 1 and IPB 5 x IPB 2 had superior characters  ideotype similar to IPB 1, IPB 3 and IPB 8 genotypes.

Keywords: Carica papaya, hermaphrodite, female, dendrogram, scatter diagram, ideotype

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Abstract. Xanthone in mangosteen fruit hull has antioxidant activities. The objective of this experiment was to determine xanthones content, physical and chemical characters, and antioxidant potentials of several groups of mangosteens qualities. The experiment was carried out from January 2008 to August 2008. The research used a randomized complete block design with four replications. The treatments were four physical fruit conditions: large size mangosteens (# 100 g), small size mangosteens (min. 50 g), yellow sap dotted fruits, and scabbed fruit. The parameters being observed were physical and chemical characteristics, xanthone content, and radical scavenging activity of mangosteen fruit hull extract. The results demonstrated that the physical condition of fruit signifi cantly affected physical fruit hull characters and chemical fruit characters. Characters of fruit hull, thickness, fresh weight, and dry weight, differed among fruit qualities. The physical condition of fruit hull also signifi cantly infl uenced total soluble solid and vitamin C content, but did not affect total titrable acid and degree of acidity of the fruits. Fruit hulls of all groups of mangosteen qualities had similar antioxidant properties with the IC50 value of 5.57-6.11 ppm.

Keywords: benzophenone, scab, vitamin C, yellow sap

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Abstract. Eggplant, yard long bean, and kangkong, were grown with polyethylene mulch with a drip irrigation system on Podzolic soil with low pH (4.5), low C-Organic (0.97%), very low total-N (0.17 %), low K content (0.15 me (100 g)-1), but had a high soil P2O5 concentration (13.7 ppm) to evaluate the best crop management practices under this soil condition. Combination of polyethylene mulch treatments (with and without), fertilizer (with and without), number of irrigation lines (0, 1, and 2 lines), and methods of fertilizer applications (preplant, split, and drip) were arranged in a Randomized Completely Block Design with four replications. The results showed that the use of mulch and fertilizer signifi cantly increased yield of all crops. One line irrigation system also resulted in the best yield. Preplanting application of 100% P, 50% N and K in addition to 50% N and K applied 10 times weekly was the best methods to produce Eggplant, Yard Long Bean, Kangkong under polyethylene mulched and drip irrigation system.

Keywords: Solanum melongena L, fertilization, fertigation, Ipomoea reptans L, micro-irrigation, polyethylene-mulched, Vigna unguilata L.

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Abstract. Biological control of bacterial wilt pathogen (Ralstonia solanacearum) of tomato using endophytic bacteria is one of the alternative control methods to support sustainable agriculture. This study was conducted to select and characterize endophytic bacteria isolated from healthy tomato stems and to test their ability to promote plant growth and suppress bacterial wilt disease. Among 49 isolates successfully isolated, 41 were non-plant pathogenic. Green house test on six selected isolates based on antagonistic effect on R. solanacearum or ability to suppress R. solanacearum population in dual culture assays obtained BC4 and BL10 isolates as promising biocontrol agents. At six weeks after transplanting, plants treated with BC4 isolate showed significantly lower disease incidence (33%) than that of control (83%). Plants height was not significantly affected by endophytic bacterial treatments. Based on 16S rRNA sequence, BC4 isolate had 97% similarity with Staphylococcus epidermidis (accession number EU834240.1), while isolate BL10 had 98% similarity with Bacillus amyloliquefaciens strain JK-SD002 (accession number AB547229.1).

Key words: biological control, endophytic bacteria, Ralstonia solanacearum, Staphylococcus epidermidis, tomato

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Abstrak. Seleksi dan identifikasi merupakan langkah penting dalam mendapatkan agens hayati untuk pengendalian penyakit tanaman. Penelitian ini bertujuan mendapatkan isolat bakteri antagonis yang potensial mengendalikan penyakit hawar pelepah padi. Penelitian dilaksanakan di laboratorium bakteriologi tanaman dan rumah kaca, Departemen Proteksi Tanaman IPB, Bogor, sejak bulan Mei 2010 sampai Pebruari 2011. Penelitian menggunakan rancangan acak lengkap dengan perlakuan isolat bakteri hasil isolasi dari beberapa lokasi ekosistem. Sebanyak 30 isolat dari 144 isolat bakteri yang diproleh bersifat antagonis terhadap penyebab penyakit hawar pelepah padi (R. solani) di tingkat in vitro. Hasil pengujian di tingkat in vivo, ternyata tiga isolat dari 30 isolat bakteri antagonis tersebut menunjukkan penekanan signifikan terhadap perkembangan penyakit hawar pelepah, yaitu isolat TT47, SS19, dan BR2. Penekanan penyakit pada perlakuan isolat bakteri TT47, SS19, dan BR2 berturut-turut sebesar 79,6%, 56,4%, and 49,4%, indeks penyakit sebesar 1,7, 3,7, dan 4,3 serta kejadian penyakit 33,3%, 73,3%, dan 80%. Berdasarkan hasil identifikasi sekuens 16S rRNA ternyata isolat SS19, TT47, dan BR2 secara berturut-turut diidentifikasi sebagai Serratia marcescens, Ralstonia pickettii, dan Bacillus subtilis.

Kata kunci: bakteri antagonis, agens pengendali hayati, Rhizoctonia solani, hawar pelepah padi.

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Abstract. The objectives of the research were to study the diversity of leaf endophytic fungi of chili, and investigate its potency in protecting host plants against Aphis gossypii Glov. Endophytic fungi were isolated from chili leaves with two categories: aphid infested plants and aphid-free plants, collected from farmer’s field in Bogor, West Java. Abundance of each fungal species from leave samples was determined by calculating frequency of isolation. The isolated fungi
were tested on population growth of A. gossypii. The fungal isolates showed suppressing effect in population growth test, was further tested on biology attributes i.e. life cycle, fecundity and body length. Five species of leaf endophytic fungi of chili were found i.e. Aspergillus flavus, Nigrospora sp., Coniothyrium sp., and SH1 (sterile hypha 1), SH2 (sterile hypha 2). Eventhough the number of endophytic fungi species in aphid-free and aphid-infested plant was same, the abundance of each species was different. Nigrospora sp., sterile hyphae 1 and sterile hyphae 2 was more abundant in aphid-free plants, but there was no difference in dominance of Aspergillus flavus and Coniothyrium sp. Nigrospora sp., SH1 and SH2 treatment reduced significantly fecundity of A. gossypii. Only SH2 treatment significantly prolonged life cycle and suppress body length, therefore the fungus had the strongest suppressing effect on population growth among fungi tested. The abundance and dominance of endophytic fungal species has relation with the infestation of A. gossypii in the field.

Key words: leaf endophytic fungi, chili, biological control, resistance, Aphis gossypii

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