Abstract. Information on genetic variability and correlation between quantitative characters with yield are important for support the selection program. The objective of the research was to estimate the genetic variability, heritability, and path analysis on agronomic characters to determine the selection criteria in the chili. This research was conducted at Research Station of Leuwikopo, Darmaga, Bogor from November 2009 until May 2010. This research observed all populations i.e. F5 population: 320 plants, IPB C2 population: 20 plants, and IPB C5 population: 20 plants. The results showed that total fruit weight, thick and fruit diameter, middle fruit diameter, blossom end fruit diameter, fruit weight, and days to flowering have a high broad sense heritability . High coefficient of genetic variability values were obtained ini number of fruits per plant, fruit weight, stem diameter, fruit diameter, and fruit weight. Based on the heritability, genetic variability, correlations analysis and path analysis, characters that can be used as selection criteria in this study is the number of fruit per plant, fruit weight, and fruit diameter.

Key words: genetic variability, heritability, path analysis, selection

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Abstrak. Serangan penyakit malaria di Indonesia terus meningkat. Di lain pihak, penyebab penyakit ini yaitu Plasmodium falciparum telah resisten terhadap obat malaria yang selama ini digunakan. Artemisinin, dari tanaman artemisia telah diteliti dapat mengendalikan malaria. Permasalahan yang dihadapi adalah kandungan artemisinin dari Artemisia annua yang ditanam di Indonesia masih sangat rendah, yaitu berkisar 0.1 — 0.5 %. Peningkatan keragaman genetik artemisia dengan menggunakan iradiasi sinar gamma merupakan metode alternative untuk mengatasi masalah tersebut. Pada penelitian sebelumnya telah dilakukan induksi mutasi pada biji artemisia dengan dosis 10-100 Gy. Planlet hasil iradiasi yang mempunyai perakaran yang baik, diaklimatisasi di rumah kaca dan galur mutan yang didapatkan ditanam di Kebun Percobaan Gunung Putri, Balittro dengan ketinggian 1545 m dpl. Sebagai pembanding digunakan tanaman yang berasal dari biji (kontrol biji) dan tanaman dari kultur in vitro yang tidak diradiasi (kontrol in vitro). Hasil penelitian menunjukkan bahwa galur-galur mutan memperlihatkan keragaman morfologi, antara lain tinggi tanaman, bentuk daun, umur berbunga. Galur mutan umumnya berbunga lebih lambat dibandingkan tanaman kontrol. Sepuluh galur mutan telah terseleksi berdasarkan bobot basah dan bobot kering tanaman yang kemudian di analisis kandungan artemisininnya. Hasil penelitian menunjukkan kandungan artemisinin dari galur mutan bervariasi antara 0.44 — 1.41%, sedangkan kandungan artemisinin dari tanaman kontrol in vitro adalah 0.43%.

Kata kunci : Artemisia annua L, artemisinin, induksi mutasi, galur mutan

Selengkapnya

Abstract. One method to obtain genetic information is the diallel cross analysis. The objective of this study was to evaluate the genetic parameters of six inbred pepper (Capsicum annuum L.) using full diallel crosses. The experiment was conducted at IPB Experiment Field, Cikabayan, Darmaga. The design was randomized complete block design (RCBD) using three replications as blocks. Data from generation F1 and parents were analyzed using the Hayman Method. Results indicated that no epistatic effects were significant for all the traits assessed. Additive genetic effects were larger than the dominant effects for yield per plant, fruit length, and diameter fruit traits. Dominant genetic effects were larger than the additive effects for fruit weight traits. Narrow-sense and broad-sense heritability were high for all the traits assessed. The character of the yield per plant, fruit weight and fruit diameter shows that there were more dominant genes in the parents. There were more recessive genes in parents for the fruit length character. IPB C7 parent was the most recessive genes containing control characters in the yield per plant. In the new improved varieties of high yielding, IPB C7 could be crossed with IPB C9. Employing individual or mass selection breeding should be successful in developing high-productivity lines in this population.

Key words: pepper, additive effects, dominant effects, yield component, full diallel

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Abstract. The objective of the study was to identify the combining ability of several introduced and local chilli genotypes and heterosis effects of their offsprings, as a result of the hybridisation process. This information will be used for production of hybrid varieties. The study was conducted from November 2006 to May 2007 at Tajur experimental station, Bogor, West Java, Indonesia. The fi ve parents with their ten F1 hybrids were evaluated in a randomized complete block design (RCBD) with three replications. The results showed that hybrid IPB C15 x IPB C19 had the highest fruit weight per plant, and hybrid IPB C8 x IPB C19 had the highest heterosis for this character. Hybrid IPB C8 x IPB C15 has highest number of fruits per plant and showed heterosis effects. Crosses among introduced and local genotypes resulted a high heterosis values. Additive variance of weight and number of fruits per plant were greater than the dominant variance. IPB C19 had the best general combining ability for fruit weight per plant character.

Keywords: capsicum, combining ability, heterobeltiosis, heterosis

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Abstract. Artemisia annua L., a family member of Asteraceae, is medicinal plants originated from China. The plant has been widely used by the local people for malaria remedy. Its active substance, artemisine, has been proved to hamper the malaria bacteria incubation, Plasmodium sp. In accordance with the WHO recomendation, the Department of Health of Indonesia is now in the attempt of developing this plant as the subtitute of chloroquin because of the malaria bacteria resistance to this antidote. In Indonesia, the artemisine content of the plant less than 0,5% is the crucial problem leading no investors are interested in its economic value. Therefore, Indonesian Medicinal and Spice Crops Research Institute; BPTO Tawangmangu, Indonesian Institute of Sciences; and PT Kimia Farma cooperate for obtaining the prime clone by breeding, selection, as well as environmental adaptation. In coping with the problem, ICABIOGRAD in the collaboration with Bogor Agricultural University have conducted the research for genetic improvement through mutative induction and field selection. This research on somaclonal variation. was conducted from Januari 2006 to Juni 2008. Eksplan used for experiment were shoots radiated with 10-100 Gy gamma ray. The result showed that the shoot radiated with the dosage of 70-100 Gy was unable to grow. On the other hand, the high level of multiplication was acquired in the one radiated with 10-30 Gy. The optimum radiation for somaclonal radiation was eventually gained with 40-60 Gy. The somaclone lines with 10-60 Gy radiation have been aclimatized and planted in Gunung Putri plot in the elevation of 1545 asl. Artemisinin content at the high biomases genotype is 0,49-0,52%.

Key words: Artemisia annua L., artemisinine, somaclone, varieties, gamma ray.

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